Work

Leuconostoc mesenteroides M13: optimization of bioprocesses and antioxidant property for food industry application

Year: 2025

Type: article

Abstract: Several reactive oxygen species are related to physiological functions but also play roles in the development of certain human diseases. Reactive oxygen species are counterbalanced by a physiological defense network such as antioxidants. Probiotic strains have been used as natural antioxidants, but its viable scalability is a challenger. The objective of this work was to optimize the bioprocesses of Leuconostoc mesenteroides M13 and evaluate its antioxidant activity and its shelf-life. The optimized production of M13 biomass was 2,542% greater than non-optimized processes, resulting in a yield of 2.67 × 1010 CFU mL-1 under conditions comprising 13.67 g L-1 of yeast extract and .73 g L-1 of magnesium sulfate. The aggregated spherical structure observed in scanning electron microscopy may have a certain protective effect. The viability of the lyophilized strain storage at 4°C was maintained for a period of up to 8 months. Antioxidant activity assays revealed efficacies for intact and lysed cells as 81 and 65%, respectively. The survival rate of the M13 strain in the presence of hydrogen peroxide was observed at 86% (1.0 mM for 8 h). M13 demonstrated a cost-effective strategy for probiotic application in the food industry as an antioxidant supplement.

Source: Food Science and Technology

DOI: 10.5327/fst.00423