Functional characterization of the CfPKS gene in drug resistance and stress responses of Colletotrichum fructicola
Year: 2025
Type: article
Abstract: Natural rubber is a critical strategic resource with substantial economic value, primarily sourced from rubber trees ( Hevea brasiliensis ). However, rubber tree production is threatened by a range of plant diseases. Colletotrichum spp. are among the most destructive pathogens causing leaf diseases in rubber trees . The widespread emergence of drug-resistant strains, resulting from the prolonged use of chemical agents, has made understanding the mechanisms of drug resistance in pathogenic fungi and developing new therapeutic approaches a major research priority. In this study, the Colletotrichum fructicola HD-1 strain was isolated from the leaves of rubber trees , and transcriptome data, profiled following treatment with the Bacillus velezensis HN-2 n-butanol extract, reveals that the CfPKS gene is implicated in drug resistance. Further analysis showed that deletion of the CfPKS gene led to a reduction in spore yield, a significant increase in chitin and melanin content, and enhanced stability of the fungal cell wall and membrane. Furthermore, the CfPKS gene deletion triggered a stronger autophagic response, with a marked upregulation of autophagy-related gene expression. This study provides valuable insights into the antibacterial mechanisms of B. velezensis biocontrol agents and the drug resistance mechanisms of C. fructicola , laying a theoretical foundation for the biological control of rubber tree anthracnose and further understanding of its drug resistance mechanisms.
Source: Industrial Crops and Products
Authors: Xiao Pan, Linglong Chu, Wenbo Liu, Weiguo Miao, Qingbiao Xie, Pengfei Jin
Institution: Hainan University
Language: English
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,720
OpenAlex ID: W4410360633