Semi-automated analysis of cerebral capillary red blood cell velocities allows modeling of transit time distribution after experimental subarachnoid hemorrhage in mice
Year: 2025
Type: article
Abstract: SignificanceMicrovascular dysfunction stems from the origin of various neurological diseases. Among these, delayed cerebral ischemia following subarachnoid hemorrhage (SAH) is a major complication. Even though pathogenesis remains poorly understood, hypotheses converge toward early and persistent microvascular dysfunction. In this context, mathematical models have been developed to study oxygen delivery using theoretical distributions of capillary flux. However, these distributions lack experimental validation.AimWe propose experimental recording of capillary red blood cell (RBC) velocities in a superficial cortical microvascular network in a mouse model of SAH, testing theoretical transit time distributions and their implications on tissue oxygenation.ApproachWe performed optical recording of RBC velocities. We propose a complete software, available on GitHub, for velocity semi-automated measurement. Experimental data were fitted with Gamma and Cauchy probability distribution functions (PDFs). Corresponding maximal oxygen metabolic rates (CMRO2max) were computed.ResultsData showed that transit time distributions changed after SAH, such that they followed a Cauchy distribution. Corresponding CMRO2max maps showed a malignant capillary heterogeneity state.ConclusionsWe provide distributions of transit times in an SAH mouse model, allowing us to discuss PDF implications for maximal oxygen consumption.
Source: Neurophotonics
Language: English
Subfield: Neurology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $1,675
OpenAlex ID: W4410386756