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Bioactivity of autotrophic and mixotrophic Tetraselmis chuii (CCAP 66/21B) extracts against H2O2-mediated stress in A549 cells.

Year: 2025
Type: article
Abstract: The trophic regime experienced by microalgae can influence their accumulation of antioxidant products such as phenolic compounds. This study investigated the antioxidant capacity and bioactivity of Dulbecco's phosphate-buffered saline (DPBS) extracts from the marine chlorophyte Tetraselmis chuii (CCAP 66/21B) cultivated under autotrophic and mixotrophic regimes. Compared to autotrophic conditions, mixotrophic cultivation significantly increased biomass yield (6.0-fold), soluble protein yield (5.7-fold) and Trolox-equivalent antioxidant capacity as measured by Folin-Ciocalteu (6.8-fold), DPPH (42.2-fold) and ABTS (15.3-fold) assays. For bioactivity, DPBS extracts (normalised by soluble protein content) were examined for their protective effects against hydrogen peroxide (H 2 O 2 )-mediated apoptosis in pulmonary carcinoma epithelial cells (A549). Both autotrophic and mixotrophic-derived extracts exhibited a dose-dependent response in H 2 O 2 -stressed A549 cells, with significant cytoprotection observed at higher concentrations (200 µg soluble protein mL -1 ), while lower concentrations were insufficient to mitigate oxidative damage and were associated with reduced cell viability. These effects involved modulation of apoptosis-related mRNA expression ( BCL2 and BAX ). The bioactive content of both autotrophic and mixotrophic-derived extracts exhibited similar biological activity with properties that may be beneficial in pulmonary pharmacology. Importantly, mixotrophy significantly upregulated the bioactive content of T. chuii (CCAP 66/21B) compared to obligate autotrophy (34.2-fold accounting for soluble protein yield and biomass generation).
DOI: 10.1016/j.procbio.2025.05.007
Language: English
Field: Energy
Open Access status: hybrid
Estimated APC paid: $3,970
OpenAlex ID: W4410397290
FWCI: 0.556
Cites: 48
Cited by: 4
Related to: 10