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Development and validation of an environmentally friendly fluorescence quenching method for linagliptin quantification using eosin Y: optimization via design of experiment and comparative greenness assessment

Year: 2025

Type: article

Abstract: , and accuracy of 99.59 ± 1.360%, in accordance with ICH guidelines. Selectivity was confirmed by the lack of interference from common pharmaceutical excipients and endogenous plasma components. The eosin Y-based fluorescence quenching method was successfully applied for the determination of linagliptin in pharmaceutical dosage forms and spiked human plasma samples. Statistical comparison of the proposed method with the reported HPLC-UV method revealed comparable analytical performance as evident by non-significant differences in the accuracy and precision profiles as well as interval equivalence testing. Furthermore, a comprehensive assessment of the environmental impact and analytical practicality of the proposed method was conducted, confirming its "green" and "blue" analytical profile. These findings establish the eosin Y-based fluorescence quenching method as a viable and environmentally friendly alternative for the routine analysis of linagliptin in various pharmaceutical and bioanalytical applications shedding light on the potential of spectrofluorometric techniques in green analytical chemistry and bioanalysis.

Source: RSC Advances

DOI: 10.1039/d5ra01945a

Authors: Saud Alqahtani, Ali Alqahtani, Taha Alqahtani, Adel Al Fatease, Ahmed A. Almrasy

Institutions: King Khalid University, Al-Azhar University

Language: English

Topic: Analytical Methods in Pharmaceuticals

Subfield: Analytical Chemistry

Field: Chemistry

Domain: Physical Sciences

SDG: Responsible consumption and production

Open Access status: gold

Estimated APC paid: $1,042