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Protocol for isolating, culturing, and artificially infecting the Arsenophonus endosymbiont of Nilaparvata lugens

Year: 2025

Type: article

Abstract: Endosymbionts play a crucial role in insect physiology and adaptation. Here, we present a protocol for isolation, cultivation, and artificial infection of the Arsenophonus endosymbiont of Nilaparvata lugens . We describe steps for symbiont isolation, in vitro culture maintenance, genetic modification, and host reinfection. This protocol enables controlled studies of symbiont-host interactions and provides a foundation for symbiont-based pest management strategies. For complete details on the use and execution of this protocol, please refer to Cai et al. 1

Source: STAR Protocols

DOI: 10.1016/j.xpro.2025.103833

Authors: Tingwei Cai, Hu Wan

Institutions: Huazhong Agricultural University, State Key Laboratory of Agricultural Microbiology

Language: English

Topic: Insect symbiosis and bacterial influences

Subfield: Insect Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: gold

Estimated APC paid: $1,550