Year: 2025
Type: article
Study design: Observational Study
Abstract: Presence of tertiary lymphoid organs within the sinuses of CRS patients are associated with a bacterial dysbiosis.• The dysbiosis is characterized by increased Staphylococcal and significantly reduced Corynebacterium species.• Mucosa with TLOs has increased expression of leukocyte chemotaxis genes. Materials and MethodsResearch involving human tissue was approved by the Human Research Ethics Committees from both The Queen Elizabeth Hospital and The University of Adelaide.Microbiome swabs and sinonasal tissue were harvested intraoperatively.Samples were collected in a standardized fashion prior to any surgical alteration.Endoscopically guided microbial swabs, nasal polyps and mucosal tissue were taken from the middle meatus.TLOs were confirmed via immunofluorescence using a previous protocol described by Lau et al. [3]. Microbiome AnalysisMicrobiome analysis was conducted utilizing a standardized protocol described in the International Sinonasal Microbiome Study (ISMS) [6], Bioinformatics was performed utilizing QIIME 2 (ver-Abbreviations: CRS, chronic rhinosinusitis;
DOI: 10.1002/alr.23608
Language: English
Subfield: Otorhinolaryngology
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $4,100
OpenAlex ID: W4410409451