Identification of S-locus F-box Protein Sequences in Diploid Potato, Solanum okadae, via Degenerate PCR
Year: 2025
Type: article
Abstract: . Key features • This protocol is applicable when the exact nucleotide sequence of the target DNA is not known but can be deduced from an amino acid sequence. • Straightforward, cost-effective, and can be used to find "new" genes or gene families. • Guidelines and a systematic approach for designing degenerate primers, along with a framework for annotating and comparing SLF genes within the Solanaceae family.
Source: BIO-PROTOCOL
Author: Amar Hundare
Institution: University of Nottingham
Language: English
Topic: Plant Reproductive Biology
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W4410412674