Work

Rapid and sensitive determination of Fluoroquinolones in tilapia using a cost-effective UPLC-MS/MS method based on dummy molecularly imprinted polymers

Year: 2025

Type: article

Abstract: This study developed a novel dummy molecularly imprinted polymer (DMIP) via emulsion polymerization for rapid, sensitive, and simultaneous detection of four fluoroquinolone (FQ) antibiotic residues in tilapia. The DMIP, coupled with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), was thoroughly characterized for its structure and adsorption properties. Optimized parameters revealed a rough surface morphology with aggregated spherical structures (400-500 nm). Compared to a non-imprinted polymer (NIP), the DMIP exhibited faster adsorption kinetics and enhanced selectivity towards enrofloxacin and related FQ compounds. The DMIP-UPLC-MS/MS method achieved satisfactory recoveries (77-105%) for the four target FQs in tilapia, with intra-day and inter-day relative standard deviations of 3.6-9.2% and 4.0-11.7%, respectively. Method detection limits (LODs) ranged from 0.02-0.06 µg/kg, while quantification limits (LOQs) ranged from 0.06-0.2 µg/kg. This novel method demonstrates excellent potential for highly sensitive and selective monitoring of trace FQ residues in aquatic food products.

Source: Talanta Open

DOI: 10.1016/j.talo.2025.100474

Authors: Xiao-Chen Huang, Shou-Lian Wei, Su Yao, Yu-Tong Lin, Jin-Kui Ma

Institutions: Zhaoqing University, Ministry of Agriculture and Rural Affairs, Guangdong ATV College of Performing Arts

Language: English

Topic: Analytical chemistry methods development

Subfield: Analytical Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $2,190

Funder: Department of Education of Guangdong Province