Work

Organelles Ca2+ redistribution contributes to cadmium-induced EMT of renal cancer cells through p-cPLA2-mediated arachidonic acid release

Year: 2025

Type: article

Abstract: Cadmium ion (Cd 2+ ) is a non-essential metal that can increase cancer risk, including potentially renal cell carcinoma (RCC), though this link is not definitive. Cd 2+ exposure impairs fatty acid metabolism in the kidneys, particularly affecting arachidonic acid (AA) levels, which are crucial for health. Previous studies have suggested that Cd 2+ -altered the AA metabolism associates with renal dysfunction. However, the role and mechanism of Cd 2+ -regulated AA source in promoting RCC progression are still unclear. This study aims to investigate how Cd 2+ exposure affects AA levels in renal cancer cells and its role in promoting cell migration. Cd 2+ exposure increases AA levels through cPLA 2 -mediated release. It also induces calcium ion (Ca 2+ ) redistribution from the endoplasmic reticulum (ER) to mitochondria, activating the p38 MAPK/cPLA 2 signaling pathway, and epithelial-mesenchymal transition (EMT) of Caki-1 cells. Cd 2+ -induced ER Ca 2+ release, p38 MAPK/cPLA 2 signaling activation, AA levels, and EMT of Caki-1 cells were effectively reversed by siRNA knockdown of IP3R. Both exogenous AA treatments and Cd 2+ -induced AA metabolite PGD2 promoted EMT and cell migration of Caki-1 cells. This study highlights Cd 2+’ s impact on fatty acid metabolism and organelle function in renal cancer cells, identifying potential therapeutic targets for RCC.

Source: Ecotoxicology and Environmental Safety

DOI: 10.1016/j.ecoenv.2025.118317