Exercise induces Mettl14 in iWAT but regulates browning and metabolism independently of Mettl14
Year: 2025
Type: article
Abstract: Exercise is a key driver of metabolic enhancement, and the impact of exercise on methyltransferase-like14 protein (Mettl14)-mediated N 6 -methyladenosine (m 6 A) modification remains unexplored.This study investigates the role of Mettl14 in inguinal white adipose tissue (iWAT) concerning exercise-induced metabolic improvement and its underlying mechanisms.We examined voluntary wheel-running exercise in C57BL/6N mice and Mettl14 heterozygous knockout (HET) mice models.We assessed metabolic phenotyping and molecular responses through body composition analysis, blood profiles, indirect calorimetry, real-time PCR, immunoblotting, and immunohistology.m 6 A levels were significantly elevated in the iWAT of trained mice, a result of increased Mettl14 expression.Additionally, higher Mettl14 levels induced by exercise were positively associated with browning markers in iWAT.Mettl14 HET led to increased weight gain and fat accumulation in a mechanism dependent on m 6 A levels.Furthermore, HET mice demonstrated notable reductions in oxygen consumption and energy expenditure at baseline.m 6 A levels were notably reduced in the iWAT of exercise-induced HET mice, yet the associated metabolic impairment was significantly mitigated.Exercise substantially correlates with enhanced browning and metabolic improvements by modulating m 6 A levels through Mettl14 expression in iWAT.However, this pathway does not critically regulate exercise-induced browning and the enhancement of whole-body metabolism.
Source: BMB Reports
Authors: Hye Jin Kim, Je Kyung Seong
Language: English
Subfield: Physiology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $550
OpenAlex ID: W4410478187