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Regulatory effects of Lactiplantibacillus plantarum on the absorption of short chain fatty acids by intestinal epithelial Caco-2 cells

Year: 2025

Type: article

Abstract: Short-chain fatty acids (SCFAs) are beneficial metabolites of intestinal microbiota. The regulation of Lactiplantibacillus plantarum on the uptake of SCFAs (acetic, propionic, and butyric acids) and their transporters by intestinal epithelial Caco-2 cells were investigated. Results indicated that the absorption of SCFAs by cells was significantly increased by L. plantarum f2, f28, and f5 ( P < 0.05); but there are significant differences between L . plantarum f19 and f16 in regulating cells absorption of SCFAs ( P < 0.05). The protein and mRNA expression levels of monocarboxylate transporter 1, Na + /H + exchanger 2 (NHE2), NHE3, and putative anion transporter 1 transporters in cells were significantly regulated by surface proteins (SP) of f19 and f16 ( P < 0.05); meanwhile, there are also significant differences between the SP of f19 and f16 in regulating transporters ( P < 0.05). L. plantarum f19 and f16 improve the absorption of SCFAs by intestinal epithelial Caco-2 cells by regulating the expression of SCFAs transporters through their SP.

Source: Journal of Functional Foods

DOI: 10.1016/j.jff.2025.106896