Work

Oxygen nanobubble-induced hyperoxia: Effects on growth, digestive enzyme activity, intestinal morphology, and biochemical parameters in kuruma prawn (Penaeus japonicus)

Year: 2025

Type: article

Abstract: Oxygen nanobubble (NB-O 2 ) technology constitutes an efficient and stable approach for the release of dissolved oxygen (DO), frequently leading to a substantial elevation in DO levels within the water. Nevertheless, the potential effects of long-term hyperoxia induced by NB-O 2 exposure in farmed shrimp remain poorly understood. Accordingly, this research aims to investigate the impact of hyperoxia during NB-O 2 treatment on growth performance, digestive enzyme activity, intestinal morphology, muscle proximate composition, and target gene expression in kuruma prawn ( Penaeus japonicus ). The present study employed NB-O 2 as a technical strategy to regulate dissolved oxygen levels and conducted a 40-day aquaculture experiment under DO conditions of 7.45 ± 0.16 mg/L (air treatment) and 15.95 ± 0.11 mg/L (NB-O 2 treatment). The study demonstrated that hyperoxia during NB-O 2 treatment enhances the growth performance of P. japonicus (weight gain rate, specific growth rate, and molting rate), improves intestinal digestive enzyme activity (pepsin, trypsin), and augments muscle proximate components (crude protein, crude fat). Furthermore, the NB-O 2 treatment increased serum biochemical parameters (glucose, total protein, albumin, acid phosphatase, alkaline phosphatase, and lysozyme) and upregulated the expression of digestion and antioxidant genes ( trypsin 1 , peroxidase ), and it significantly reduced the number of pathogenic microorganisms in seawater, demonstrating its effectiveness in water quality purification. In conclusion, long-term hyperoxia induced by NB-O 2 exposure offer potential benefits for the aquaculture of P. japonicus , promoting overall health and stability.

Source: Aquaculture Reports

DOI: 10.1016/j.aqrep.2025.102882