Work

eLife Assessment: Structural and dynamic impacts of single-atom disruptions to guide RNA interactions within the recognition lobe of Geobacillus stearothermophilus Cas9

Year: 2025

Type: peer-review

Abstract: Solution biophysics defines the influence of local dynamics and allosteric regulation on guide RNA binding affinity and DNA cleavage specificity in a thermophilic Cas9 from Geobacillus stearothermophilus.

DOI: 10.7554/elife.99275.4.sa0

Author: Julien Roche

Institution: Iowa State University

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

OpenAlex ID: W4410501495

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