Immune efficacy of oprH chitosan nanoparticle DNA vaccine against Pseudomonas aeruginosa.
Year: 2025
Type: article
Abstract: Background: is a zoonotic pathogen that poses a threat to human and animal health. However, no vaccine exists for controlling this bacterium. Aims: . Methods: 2 weeks after the last vaccination and the survival numbers were counted until day 15 post challenge. Then, the protective rates were calculated. Results: The particle size of the chitosan nanoparticle DNA vaccine was approximately 200 nm and close to spherical; the encapsulation efficiency was 95.88%, and it could effectively resist degradation by DNase. Following vaccination, serum antibodies, stimulation index (SI) value, and concentrations of IFN-γ, IL-2, and IL-4 in chickens immunized with the chitosan nanoparticle DNA vaccine were significantly higher than those that were vaccinated with the naked DNA vaccine (P<0.05). The protective rates of poprH, CoprH, OMP vaccine, and inactive vaccine groups were 55%, 75%, 75%, and 90%, respectively. Conclusion: gene.
Source: PubMed
Institution: Henan University of Science and Technology
Language: English
Subfield: Microbiology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4410510674