Year: 2025
Type: article
Abstract: Background: Acute lymphoblastic leukemia (ALL) is a malignant disorder in both humans and animals. L-asparaginase (L-ASNase) has limitations as a chemotherapy agent due to adverse effects and low serum stability. In a previous study, L-ASNase was chemically modified with carboxymethyl dextran to enhance its properties. Aims: This study aimed to validate the potential of these modifications using the NALM-6 cell line. Methods: genes (important genes in autophagy), with data analysis conducted using PRISM software. Results: The modified L-ASNase reduced the viability of NALM-6 cells and induced higher levels of apoptosis (P=0.001). Interestingly, the modified enzyme had a lesser impact on autophagy, which is important for avoiding treatment resistance. Conclusion: The modified L-ASNase showed enhanced effectiveness in reducing the viability of NALM-6 cells and induced higher levels of apoptosis. Interestingly, the modified enzyme had a lesser effect on autophagy, which is important as excessive autophagy can lead to treatment resistance. These findings suggest that the modified L-ASNase may have the potential to be a more effective chemotherapeutic agent for ALL treatments.
Source: PubMed
Institutions: Shiraz University, Shiraz University of Medical Sciences
Language: English
Field: Medicine
Domain: Health Sciences
Open Access status: green
Funder: Shiraz University
OpenAlex ID: W4410510689