Work

Physicochemical and functional assessment of messenger RNA 5′Cap-end impurities under forced degradation conditions

Year: 2025

Type: article

Abstract: using model mRNAs with elevated impurity levels. mRNA 5'Cap degradation impurities significantly impacted protein expression but did not trigger innate immune response pathways. 5'Cap degradation impurities should be considered as a critical quality attribute of mRNA therapeutics and should be monitored during production, purification, formulation, and storage.

Source: Molecular Therapy — Nucleic Acids

DOI: 10.1016/j.omtn.2025.102570