Hybrid of glucose oxidase and enzyme-like Cu&Ce: Efficient glucose scavenging and generation of hydroxyl, hydroperoxyl, and superoxide radicals for wound healing
Year: 2025
Type: article
Abstract: ABSTRACT Glucose oxidase (GOD) is a well-known flavin adenine dinucleotide (FAD)-containing oxidase. GOD oxidizes glucose to D-glucono-δ-lactone with FAD being reduced to FADH2, and O 2 is reduced by FADH2 to hydrogen peroxide (H 2 O 2 ). To efficiently generate H 2 O 2 and subsequently produce radicals through glucose consumption, we synthesized the hybrid material GOD@Cu&Ce by encapsulating GOD with Cu II and Ce IV ions in phosphate buffer saline (PBS). Owing to the synergistic cooperation between Cu II and Ce IV , hydroxyl (•OH), hydroperoxyl (HOO•), and superoxide (O 2 •- ) radicals are efficiently generated via H 2 O 2 decomposition by Cu&Ce. Concurrently, O 2 is produced, demonstrating that Cu&Ce exhibits catalase-like activity. The in situ -generated O 2 facilitates the oxidation of FADH2 back to FAD, thereby enhancing GOD activity. The efficient and sustained generation of •OH, HOO•, and O 2 •- radicals from glucose conversion arises from two mechanisms: (1) synergistic H 2 O 2 decomposition by enzyme-mimicking Cu&Ce, and (2) in situ O 2 production that boosts GOD activity. Studies on the mechanisms reveal that Cu II and Ce IV cooperatively promote the generation of •OH, HOO•, O 2 •- , and O 2 . GOD@Cu&Ce combines rapid glucose consumption with high radical yield. In wound healing assays, this hybrid material demonstrates potent antibacterial activity.
Source: Materials Today Bio
Authors: Yining Han, Jiaxing Wei, Wei Feng
Institution: Beijing University of Chemical Technology
Language: English
Subfield: Materials Chemistry
Field: Materials Science
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $3,400
Award: G5763442329
OpenAlex ID: W4410540885