Histopathological evaluation of trachea, lung, and mesonephros in specific pathogen free-eggs embryos inoculated for titration of avian infectious bronchitis virus M41 strain.
Year: 2025
Type: article
Abstract: , from each dilution 0.10 mL is used. The aim of this study was to determine the effects of the avian bronchitis virus injected into the allantoic cavity of ten days old embryonated eggs. Real-time polymerase chain reaction tests determined the viral load in the allantoic fluid. The embryos were removed to study gross injuries. The trachea, lung, and mesonephros were removed and submitted for histopathological studies, and nuclear factor-kappa B immunofluorescence analysis. The results revealed that the dilution of one-thousandth of the virus in the embryos caused the highest organ damage and viral replication. Varying degrees of hyperemia, edema, cellular infiltration, and degeneration were observed in the trachea, lung, and mesonephros depending on the virus dilution. This study provides valuable insights into the pathogenesis of the avian bronchitis virus, and has a potential impact on achieving an effective vaccine.
Source: PubMed
Authors: Farhad Farhadi, Amir Abbas Farshid, Mohammad Hossein Alavi, Ali Ameghi, Seyede Soraya Mahmoudi
Language: English
Subfield: Animal Science and Zoology
Domain: Life Sciences
Open Access status: green
Funder: Urmia University
OpenAlex ID: W4410548780