Dual-comb mid-infrared spectromicroscopy with photothermal fluorescence detection
Year: 2025
Type: article
Abstract: An approach is described for spectrally parallel hyperspectral mid-infrared imaging with spatial resolution dictated by fluorescence imaging. Quantum cascade laser (QCL)-based dual-comb mid-infrared spectroscopy enables the acquisition of infrared spectra at high speed (<1 millisecond) through the generation of optical beat patterns and radio-frequency detection. The high-speed nature of the spectral acquisition is shown to support spectral mapping in microscopy measurements. Direct detection of the transmitted infrared beam yields high signal-to-noise spectral information, but long infrared wavelengths impose low diffraction-limited spatial resolution. The use of fluorescence detected photothermal infrared (F-PTIR) imaging provides high spatial resolution tied directly to the integrated IR absorption. Computational imaging using a multi-agent consensus equilibrium (MACE) approach combines the high spatial resolution of F-PTIR and the high spectral information of dual-comb infrared transmission in a single optimized equilibrium hyperspectral data cube.
Source: Optics Express
DOI: 10.1364/oe.557991
Authors: Gwendylan A. Turner, Daniel I. Herman, Aleksandr Razumtcev, Markus Mangold, Minghe Li, Ziyi Cao, Jiayue Rong, Charles A. Bouman, Gregery Buzzard, Garth J. Simpson
Institution: Purdue University West Lafayette
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $2,270
Awards: MRI: Track 1 Development of a Dual-Comb Fluorescence-Detected Photothermal Mid-Infrared (DC-FPTIR) Microscope, Nonlinear Optical Analysis of Molecular Composition and Dynamics within Heterogeneous Assemblies, G569852198, D3SC: Nonlinear Optical Analysis of Proteins in Glasses, G7905610480, CIF: Medium: Multi-Agent Consensus Equilibrium: Modular Methods for Integrating Disparate Sources of Expertise
OpenAlex ID: W4410554048