Figure 2 from APOBEC3 Activity Promotes the Survival and Evolution of Drug-Tolerant Persister Cells during EGFR Inhibitor Resistance in Lung Cancer
Year: 2025
Type: other
Abstract: Engineering an inducible system for APOBEC3B expression in PC9 cells. A, qRT-PCR analysis showing A3A and A3B expression in PC9 cells following treatment with gefitinib or osimertinib over the course of 14 days. Error bars represent SEM of three technical replicates. Veh, vehicle. B, Schematic of Cre-inducible APOBEC3B expression. C, qRT-PCR analysis showing A3B expression in PC9 cells following infection with Cre recombinase. Error bars represent SEM of three technical replicates. *, P < 0.05. BT474 and SKBR3 cells are shown as controls. D, Western blots showing protein expression of HA-tagged A3B in PC9 cells following infection with Cre recombinase. E, In vitro deaminase activity assay in PC9 cells following infection with Cre recombinase. BT474 and SKBR3 cells are shown as controls. % deamination was calculated as described in “Materials and Methods”. F, Quantification of % deamination in two replicates of control PC9 cells (−Cre) and five replicates of A3B-expressing PC9 (+Cre). % deamination was calculated as described in “Materials and Methods”. An unpaired t test was performed to determine statistical significance. **, P < 0.005. G, Growth curves for PC9 cells expressing A3B (+Cre) or control cells (−Cre). Error bars represent SEM of two biological replicates. A two-way ANOVA was performed to determine statistical significance. ns, not significant.
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4410565957