Figure 5 from APOBEC3 Activity Promotes the Survival and Evolution of Drug-Tolerant Persister Cells during EGFR Inhibitor Resistance in Lung Cancer
Year: 2025
Type: other
Abstract: APOBEC3B-expressing PC9 cells show evidence of squamous cell transdifferentiation during acquired gefitinib resistance. A, Normalized gene counts for TP63 from RNA-seq in gefitinib-sensitive and GR PC9 cells, with or without A3B expression. B, qRT-PCR analysis showing ∆Np63 and total p63 mRNA expression in gefitinib-sensitive and GR PC9 A3B-on cells. Parental PC9 are shown as a control. Error bars represent SEM of three technical replicates. An unpaired t test was performed to determine statistical significance. ***, P < 0.0005; ****, P < 0.0001. C, Normalized gene counts for MUC5B, IL-1A, IL-1B, and KRT6A from RNA-seq in gefitinib-sensitive and GR PC9 cells expressing A3B. D, IHC images showing ∆Np63 protein expression in sensitive and resistant PC9 A3B-on cells. Insets are shown at 20× magnification. E, Quantification of ∆Np63-positive cells for sensitive and resistant PC9 A3B-on cells shown in D. Each point represents a field of view at 20× magnification. Error bars represent SEM of five fields of view. An unpaired t test was performed to determine statistical significance. ***, P < 0.0005; ****, P < 0.0001.
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4410566030