Functional ultrasound imaging and neuronal activity: How accurate is the spatiotemporal match?
Year: 2025
Type: article
Abstract: Over the last decade, functional ultrasound (fUS) has risen as a critical tool in functional neuroimaging, leveraging hemodynamic changes to infer neural activity indirectly. Recent studies have established a strong correlation between neural spike rates (SR) and functional ultrasound signals. However, understanding their spatial distribution and variability across different brain areas is required to thoroughly interpret fUS signals. In this regard, we conducted simultaneous fUS imaging and Neuropixels recordings during stimulus-evoked activity in awake mice within three regions of the visual pathway. Our findings indicate that the temporal dynamics of fUS and SR signals are linearly correlated, providing an approximate calibration between the two signals. Conversely, the spatial correlation between the two signals remains consistent across all regions with a spread of approximately 300 micrometers. Finally, we introduce a model that integrates the spatial and temporal components of the fUS signal, allowing for a more accurate interpretation of fUS images.
Source: Imaging Neuroscience
DOI: 10.1162/imag.a.34
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: diamond
Awards: Ultrasound hemodynamic neuroimaging to improve monitored management in patients with traumatic brain injury (USNI4TBI), G1395299968, G1489874680, G1527625874, Understanding brain circuit dysfunction in amblyopia using large-scale multimodal recordings in a new visuomotor task applied to animal models and patients, G2629833160, TRPM3 variants underlie neurodevelopmental disorders and epileptic encephalopathies, Relieve PAIN by manipulating BRAIN circuits (PAINBRAIN)
OpenAlex ID: W4410579017