Protocol for the decellularization of human intestinal tissue from patients with inflammatory bowel disease
Year: 2025
Type: article
Abstract: Decellularization of the human intestine in patients with inflammatory bowel disease (IBD) provides an experimental platform for the investigation of the bowel extracellular matrix (ECM). Here, we present a protocol for removing cellular components from the IBD intestinal tissue while preserving its structure and ECM components. We describe steps for pre-processing specimens and decellularization by osmotic pressure difference, followed by detergents and enzymes. We then detail procedures for extensive washing. The decellularized tissue can be stored or processed according to the experimental requirements. For complete details on the use and execution of this protocol, please refer to Lin et al. 1
Source: STAR Protocols
Language: English
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $1,550
Funders: Leona M. and Harry B. Helmsley Charitable Trust, Cleveland Clinic, Crohn's and Colitis Foundation, National Natural Science Foundation of China, National Institutes of Health, Guangzhou Municipal Science and Technology Project, National Institute of Diabetes and Digestive and Kidney Diseases
OpenAlex ID: W4410613355