Figure 5 from PD-1 Blockade Mitigates Surgery-Induced Immunosuppression and Increases the Efficacy of Photodynamic Therapy for Pleural Mesothelioma
Year: 2025
Type: other
Abstract: PDT decreases PD-L1 expression and significantly reduces tumor growth after TI. TI/PDT-treated mice had significantly longer tumor growth times (to 400 mm3) than TI mice for AB12 (P < 0.001, A) and AE17O (P < 0.001, B), with medians of 7 days for TI vs. 24 days for TI/PDT for AB12 and 13 days for TI vs. 20.5 days for TI/PDT for AE17O. In AB12 mice, n = 11 for TI and n = 16 for TI/PDT. In AE17O mice, n = 11 for TI and n = 18 for TI/PDT. C, CD11b+ Ly6G+ cells were significantly increased after TI/PDT (n = 6) compared with TI (n = 8) in AB12 tumors (P < 0.001), and their PD-L1 expression was significantly decreased following PDT (P = 0.005). D, Tumors treated with PDT (n = 6) similarly had increased Ly6G+ cells (P = 0.002) and decreased PD-L1 expression (P = 0.005) compared with TCs (n = 7). E, PDT after TI significantly decreased the proportion of Ly6G− cells (P < 0.001) and led to a significant drop in their PD-L1 levels (P = 0.008). F, The proportion of CD11b+ Ly6G− cells as a subset of CD45+ immune cells decreased significantly after PDT (P = 0.001), and their PD-L1 levels were lower on average but not significantly so (P = 0.073). G, For CD45− cells, TI/PDT led to a significant drop in the percentage of viable CD45− cells (P < 0.001) but did not alter PD-L1 expression (P = 0.30). H, PDT of CD45− cells also significantly decreased viability (P = 0.001), but no significant differences in PD-L1 expression of these cells were observed (P = 0.29). Representative gates of viable CD45− cells are shown below for each condition. I, Viable CD45− cells after TI/PDT had significantly higher levels of PD-L1 than CD45− cells after PDT alone (P = 0.004). Tumor responses (growth to 400 mm3) were compared using log-rank (Mantel–Cox) tests, and all other comparisons were made using a Mann–Whitney test. FSC-A, forward scatter area; SSC-A, side scatter area.
Authors: Gwendolyn M. Cramer, Richard W. Davis, Emmanouil Papasavvas, Astero Klampatsa, Joann M. Miller, Shirron Carter, Ruth Ikpe, Min Yuan, Sandy Widura, R. Sonali Majumdar, Sally McNulty, Mary Putt, Andrew V. Kossenkov, Luis J. Montaner, Sunil Singhal, Edmund K. Moon, Steven M. Albelda, Keith A. Cengel, Theresa M. Busch
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4410618786