Figure 4 from PD-1 Blockade Mitigates Surgery-Induced Immunosuppression and Increases the Efficacy of Photodynamic Therapy for Pleural Mesothelioma
Year: 2025
Type: other
Abstract: TI increases PD-1 expression on AB12 intratumoral T cells. A, In AB12 tumors, 2 days after TI, the fraction of CD4 and CD8 T cells (as a percentage of all CD3+ cells) was not significantly different between TI (n = 11) and TC (n = 14). However, the levels of DX5+ (NKT cells) decreased slightly but significantly in TI (P = 0.017). B, For all CD3+ cells, the levels of PD-1 were significantly upregulated by TI compared with TC (P = 0.009). C, For individual T-cell populations, PD-1 was significantly increased in CD8+ (P = 0.001) and DX5+ (P = 0.006) T cells after TI compared with TC. D, After 5 days, TC and TI tumors have similar numbers of CD8, CD4, and DX5+ T cells. For CD4 and CD8 T cells, n = 11 for TC and n = 11 for TI. For NKT cells, n = 8 for TI and n = 8 for TC. E, For all CD3+ cells, significant differences in median PD-1 levels were not detected at the 5-day time point (P = 0.098). F, CD4+ T cells had significantly higher PD-1 expression 5 days after TI (P = 0.049). Differences in PD-1 expression of CD8 and DX5 T cells at 5 days after TI were small and not statistically significant. Comparisons were made using a Mann–Whitney test. FMO, fluorscence minus one.
Authors: Gwendolyn M. Cramer, Richard W. Davis, Emmanouil Papasavvas, Astero Klampatsa, Joann M. Miller, Shirron Carter, Ruth Ikpe, Min Yuan, Sandy Widura, R. Sonali Majumdar, Sally McNulty, Mary Putt, Andrew V. Kossenkov, Luis J. Montaner, Sunil Singhal, Edmund K. Moon, Steven M. Albelda, Keith A. Cengel, Theresa M. Busch
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4410618787