Figure 1 from PD-1 Blockade Mitigates Surgery-Induced Immunosuppression and Increases the Efficacy of Photodynamic Therapy for Pleural Mesothelioma
Year: 2025
Type: other
Abstract: Mesothelioma surgery in humans and mice alters immune pathways. A, RNA-seq analysis was performed on tumor tissue isolated over the time course of surgery from 31 patients with PM. Volcano plot of significant gene expression changes associated with time of sample collection showing strength of correlation with time (Spearman rho) and significance of association based on DESeq2 algorithm. FDR <5% results are shown in red and blue with top genes by P value and/or correlation coefficients highlighted. dn, downregulated; ns, nonsignificant; up, upregulated. B, Analysis of FDR <5% genes significantly associated with the time of collection using IPA. Canonical pathways identified by IPA with FDR <5% and prediction Z-score >2 are shown. The color scale intensity, with red showing activated pathways and blue showing inhibited pathways, indicates significance of enrichment (P value). Numbers of differentially expressed genes in each pathway are indicated to the right of each bar. C, Heatmap showing log2 gene expression versus average across tumor specimens for members of the IL-6 and IL-1 families with specimens sorted by the time from initiation of surgery (green bar at the top). Specimens from the same individual patients (uniquely shaped and color-coded) are marked at the time of collection. IL-6 and IL-1 pathways both show increased activity as surgery progresses. D, In the AB12 mouse mesothelioma TI model, IL-1Ra protein levels are higher at 4 hours after TI (n = 7) compared with samples of untreated TC (n = 6, Mann–Whitney test P = 0.001).
Authors: Gwendolyn M. Cramer, Richard W. Davis, Emmanouil Papasavvas, Astero Klampatsa, Joann M. Miller, Shirron Carter, Ruth Ikpe, Min Yuan, Sandy Widura, R. Sonali Majumdar, Sally McNulty, Mary Putt, Andrew V. Kossenkov, Luis J. Montaner, Sunil Singhal, Edmund K. Moon, Steven M. Albelda, Keith A. Cengel, Theresa M. Busch
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4410618811