Macrococcus capreoli sp. nov., a new fosfomycin resistant species isolated from feces and nasal swabs of deer
Year: 2025
Type: article
Abstract: Five strains of Gram-stain-positive, non-motile, and coccoid-shaped bacteria, designated as TMW 2.2395 T , TMW 2.2628, TMW 2.2670, TMW 2.2756 and TMW 2.2757 were isolated from feces and nasal mucosa of wild living roe deer ( Capreolus capreolus ) and red deer ( Cervus elaphus ), respectively. The isolates share identical 16S rRNA gene sequences and are classified within the genus Macrococcus based on 16S and MALDI-TOF MS analyses. Phylogenetic analysis revealed that average nucleotide identity (ANI) values were below the accepted thresholds for prokaryotic species delineation, with the type strains of M. goetzii , M. bohemicum and M. epidermidis showing the highest relatedness values (79.59 %, 79.30 % and 79.52 %, respectively). Digital DNA–DNA hybridization (dDDH) values were below 23 % confirming that all five strains belong to a new species within the genus Macrococcus . All strains were catalase and oxidase positive, grew optimally at 37 °C and pH 7.0, but demonstrated considerably lower salt tolerance (< 7.5 % w / v ) compared to other species in the genus. Moreover, the data of this study suggests that members of this new species possess an intrinsic resistance to fosfomycin, putatively mediated by a fosB homologue. The genomes range from 2.4 and 2.5 Mb in size, with a G + C content of 34.8 to 35.1 %. The primary respiratory quinone of the type strain TMW 2.2395 T is Menaquinone 6 (MK-6, 98.6 %), the cell wall peptidoglycan type is A3α L-Lys-Gly 3 -L-Ser and the major fatty acids are C 14:0 , C 16:0 and C 18:0 . Phylogenetic, phenotypic and chemotaxonomic data collectively suggest that these strains represent a novel species of the genus Macrococcus , for which the name Macrococcus capreoli sp. nov. is proposed. The type strain is TMW 2.2395 T = DSM 113939 T = LMG 32618 T .
Authors: Carolin J. Schiffer, Matthias A. Ehrmann
Institution: Technical University of Munich
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $4,010
OpenAlex ID: W4410633010