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Protocol for assessing the structural architecture, integrity, and cellular composition of murine bone ex vivo

Year: 2025
Type: article
Abstract: Understanding changes in murine bone is essential to comprehend bone homeostasis and the role of therapeutics in bone diseases. Here, we present a protocol to assess the structural architecture, integrity, and cellular composition of murine bone ex vivo . We describe steps for imaging and analyzing bone by micro-computed tomography (CT) and functionally testing bone strength through three-point bending. We then detail procedures for histology and analysis of calcein labeling. For complete details on the use and execution of this protocol, please refer to Lewis et al. 1
DOI: 10.1016/j.xpro.2025.103843
Authors: Jonathan W. Lewis, Kathryn Frost, Georgiana Neag, Holly Adams, Emily Powell, Orla Gallagher, Ilaria Bellantuono, Amy J. Naylor, Helen M. McGettrick
Language: English
Field: Medicine
Open Access status: gold
Estimated APC paid: $1,550
Funders: British Society for Research on Ageing, University of Birmingham, University of Sheffield, Versus Arthritis, Medical Research Council, European Regional Development Fund
OpenAlex ID: W4410709071
FWCI: 0.918
Cites: 16
Cited by: 1
Related to: 10