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The mechanism of ECT1/E6E7 cervical intraepithelial neoplasia cells regulated by Acinetobacter lwoffii through circ-LDHA/HMGB1

Year: 2025
Type: article
Abstract: BACKGROUND: To investigate the mechanism by which Acinetobacter lwoffii-induced circ-LDHA/HMGB1 activation regulates the ability of ECT1/E6E7 cells to promote cervical intraepithelial neoplasia (CIN). METHODS: After establishing a co-culture model of Acinetobacter lwoffii and ECT1/E6E7 cells, the proliferation, migration and apoptosis of ECT1/E6E7 cells were detected by CCK8, transwell and flow cytometry assays. The relative expression levels of circ-LDHA and HMGB1 were detected by real-time quantitative PCR (qRT-PCR). RESULTS: Circ-LDHA expression was lower in normal cervical tissues and greater in the cervical cancer group and CIN group than in the normal group (P < 0.05). The optimal coculture model with Acinetobacter lwoffii was an MOI of 100 following 18 h-24 h of coculture. The proliferative activity of the ECT1/E6E7 cells and the relative mRNA expression levels of circ-LDHA and HMGB1 were significantly increased within 36h of coculture, and the apoptosis rate of the ECT1/E6E7 cells was significantly increased after 12 h of coculture. The migration rate of the ECT1/E6E7 cells increased from 12 h to 24 h (all P < 0.05). CONCLUSION: Acinetobacter lwoffii can regulate the development and progression of CIN by regulating the expression of circ-LDHA and HMGB1 in a time-dependent manner.
DOI: 10.1186/s12866-025-04043-y
Language: English
Open Access status: gold
Estimated APC paid: $2,990
OpenAlex ID: W4410723558
FWCI: 0.761
Cites: 37
Cited by: 3
Related to: 10