Work

Antibiotics in milk: Validation of analytical methodologies for ensuring food safety

Year: 2025

Type: article

Abstract: Contamination of milk with antibiotics poses a significant threat to food safety and public health. To address this issue, it is crucial to develop and validate accurate analytical methodologies that can reliably detect and quantify the presence of antibiotics in milk. This work aimed to implement an analytical methodology by UHPLC-QTRAP-MS/MS for the determination of 88 antibiotics belonging to ten pharmacological classes in milk. The method showed high selectivity and sensitivity, with linearities above 0.95 obtained through a matrix-matched calibration approach. Decision limit (CCα) values ranged from 1.3 to 239.4 μg.kg -1 . Limits of detection (LOD) and limits of quantification (LOQ) were determined with the highest values of 22.33 μg.kg -1 and 44.85 μg.kg -1 , respectively. Repeatability and reproducibility were below 21.8 and 23.2%, and recovery ranged between 80.9 and 118.9%. Application to real samples from local markets demonstrated the presence of macrolides and sulphonamides, though concentrations were below the regulated limits. These results showed the importance of developing and validating new analytical strategies to ensure the safety of dairy products.

Source: International Dairy Journal

DOI: 10.1016/j.idairyj.2025.106308

Authors: M. Leite, A. Freitas

Institutions: Instituto Nacional de Investigação Agrária e Veterinária, Rede de Química e Tecnologia, LAQV Requimte

Language: English

Topic: Pesticide Residue Analysis and Safety

Subfield: Food Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDGs: Zero hunger, Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,290