Work

Concurrent Hydrolysis–Fermentation of Tenebrio molitor Protein by Lactobacillus plantarum KCCM13068P Attenuates Inflammation in RAW 264.7 Macrophages and Constipation in Loperamide-Induced Mice

Year: 2025

Type: article

Abstract: Constipation is a common gastrointestinal disorder that negatively impacts quality of life and gut function. This study aimed to enhance the functional properties of Tenebrio molitor (mealworm) protein hydrolysates through fermentation with Lactobacillus plantarum KCCM13068P (TmLp). Compared to non-fermented hydrolysates (HeTm), TmLp exhibited improved protein hydrolysis and increased levels of amino acids associated with intestinal health. In vitro, TmLp showed anti-inflammatory effects in RAW 264.7 macrophages and promoted the growth of beneficial gut bacteria. In a loperamide-induced constipation mouse model, TmLp significantly enhanced fecal output, water content, and intestinal transit. These findings suggest that TmLp may serve as a promising functional food ingredient for relieving constipation and promoting gut health.

Source: Foods

DOI: 10.3390/foods14111886

Authors: Hyun-Sol Jo, Da-Bin Song, Sang-Hee Lee, Kyu-Shik Lee, Jungwoo Yang, Sun-Mee Hong

Institutions: Korea Food Research Institute, Dongguk University

Language: English

Topic: Protein Hydrolysis and Bioactive Peptides

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDGs: Good health and well-being, Zero hunger

Open Access status: gold

Estimated APC paid: $3,499