Work

Local Iontophoretic Application for Pharmacological Induction of Long-Term Synaptic Depression

Year: 2025

Type: article

Abstract: sensor proteins. Key features • Easy, fast, and reliable induction of LTD in primary cultured neurons using iontophoretic NMDA application. • Suitable for the application of any ionic water-soluble compound and compatible with simultaneous multicolor fluorescence imaging and electrophysiological recording. • This protocol enables pharmacological targeting of individual neurons, substantially increasing experimental throughput.

Source: BIO-PROTOCOL

DOI: 10.21769/bioprotoc.5338

Authors: Borys Olifirov, Oleksandra Fedchenko, Alexandr Dovgan, Daria Babets, Volodymyr Krotov, Volodymyr Cherkas, Pavel Belan

Institutions: Bogomoletz Institute of Physiology, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Polish Academy of Sciences, Kyiv Academic University

Language: English

Topic: Neuroscience and Neuropharmacology Research

Subfield: Cellular and Molecular Neuroscience

Field: Neuroscience

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: diamond

OpenAlex ID: W4410767130

FWCI:

Cites: 29

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Related to: 10