Work

Genotoxic and cytotoxic effect of vanadium inhalation and oral administration of Hintonia latiflora methanolic extract in mice

Year: 2025

Type: article

Abstract: INTRODUCTION: Environmental air pollution constitutes a significant problem for large cities, primarily due to anthropogenic activities such as burning fossil fuels, which have led to an escalation that release suspended particulate matter (PM);vanadium, a metal adhered to PM, leading to their entry into the bloodstream and subsequently V distribution to various organs. Research has demonstrated that exposure to vanadium (V) can induce single-strand breaks and the presence of micronuclei in cells. In addition to this, the use of herbal remedies has seen a marked increase on a global scale in recent years. It is estimated that more population makes use of these remedies without being aware of their potential health implications. Hintonia latiflora (Hl) is a herbal remedy commonly used, reported also as genotoxic. OBJECTIVE: To evaluate the genotoxic and cytotoxic effect of V inhalation and co-exposure with the administration of methanolic extract of Hl (HlMeOHe). METHOD: Twenty-five male CD1 mice distributed in four groups were used: control, V, Tween 80 % (0.04 %), HlMeOHe (250 mg/kg per day) and V+HlMeOHe (250 mg/kg per day). Animals inhaled V twice a week. Blood samples were obtained at designated time points: 0, 24 and 72 hours; 7, 14 and 21 days. Subsequently, the samples were subjected to rigorous testing procedures, including the assessment of cell viability by fluorochromes, alkaline comet assay and micronucleus assay with acridine orange. RESULTS: The inhalation of V, either as a standalone treatment or in conjunction with HlMeOHe, did not affect cell viability. Administration of HlMeOHe, did not result in the generation of single-strand breaks, a finding that contrast with the effects of V. The co-exposure of V and HlMeOHe exhibited from day 14-21 a significant decrease (p ≤ 0.05) in micronuclei assay, as well as a in single-strand breaks (p < 0.05) compared against control group. CONCLUSION: These results suggest that HlMeOHe is not cytotoxic or genotoxic maybe because of its phenolic compounds, reducing the genotoxic effect induced by V.

Source: Journal of Trace Elements in Medicine and Biology

DOI: 10.1016/j.jtemb.2025.127677

Authors: María Eugenia Cervantes-Valencia, Marcela Rojas-Lemus, Nelly López-Valdez, Adriana González-Villalva, Teresa I Fortoul

Institution: Universidad Nacional Autónoma de México

Language: English

Topic: Carcinogens and Genotoxicity Assessment

Subfield: Cancer Research

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,700