Case study of non-lethal sampling for plant-pollinator networks via barcoding and metabarcoding on bumble bees in Germany
Year: 2025
Type: article
Abstract: 1. Insect decline is threatening ecosystem stability, making information about foraging preferences of pollinators a vital piece of information to acquire. A powerful emerging tool to study pollinator foraging behaviour is pollen-metabarcoding. This usually involves lethal sampling of insects. 2. Here, we propose a new, non-lethal way of sampling DNA for the analysis of pollen loads of bumble bees as well as the pollinator. The new methodology does not significantly harm the insect and is easy to implement in a wide range of study designs. The tool is cheap and easy to acquire, can easily be used in the field and has the potential to become a powerful tool in studying plant-pollinator interactions. 3. To test its feasibility, plant-pollinator networks were analysed using metabarcoding of the ITS2 region. Plants flowering at the time of collection were also recorded as a reference comparison. 4. Bumble bees with ambiguous morphology were additionally identified, based on COI barcoding. 5. With the workflow developed here, it is possible to gain knowledge about plants and their pollinators in a non-lethal way without reducing population sizes. This makes this method particularly suitable for endangered and protected species.
Source: Metabarcoding and Metagenomics
Authors: Alexander Edwards, Birgit Gemeinholzer
Institution: University of Kassel
Language: English
Subfield: Ecology
Field: Environmental Science
Domain: Physical Sciences
SDG: Life on land
Open Access status: gold
Estimated APC paid: $701
OpenAlex ID: W4410780590