Novel 5-((Phenylimino)methyl)-1,2,4-triazol-3-one Derivatives: Synthesis, Anticancer Potential and Molecular Docking Insights
Year: 2025
Type: article
Abstract: In this study, a series of substituted 5-((phenylimino)methyl)-2,4-dihydro-3H-1,2,4-triazol-3-one derivatives (5a-o) were synthesized and evaluated for their anticancer activity against various human cancer cell lines, including A549 (non-small cell lung cancer), HCT-116 (colorectal cancer) and PANC-1 (pancreatic cancer). The compounds were synthesized through a multi-steps process involving the preparation of ethyl β-N-Boc-oxalamidrazone, followed by cyclization to form ethyl 5-oxo-4,5-dihydro-1H-1,2,4-triazole-3-carboxylate, reduction to yield 5-oxo-4,5-dihydro-1H-1,2,4-triazole-3-carbaldehyde and subsequent condensation with various substituted anilines to obtain the final derivatives. Structures of the compounds were confirmed by 1H NMR, 13C NMR and high-resolution mass (HRMS) spectral methods. Molecular docking studies targeting epidermal growth factor receptor (EGFR) and cyclin-dependent kinase 4 (CDK4) revealed that certain derivatives, particularly those with electron-withdrawing groups like -NO2, -OH and -CF3, exhibited strong binding affinities, suggesting potential as inhibitors of these targets. In vitro cytotoxicity assays showed significant antiproliferative effects, with compound 5h (4-CF3) exhibiting the highest potency against A549 (IC50: 7.80 ± 3.06 µM) and PANC-1 (IC50: 8.75 ± 1.86 µM). Compound 5j (3,4-(OCH3)2) demonstrated notable activity against A549 (IC50: 5.96 ± 1.02 µM). Structure activity relationship analysis indicated that electron-donating groups generally enhanced activity, while electron-withdrawing groups had varying effects based on their position. These findings highlight the potential of these derivatives as lead compounds for anticancer drug development. Further optimization and in vivo studies are needed to fully explore their therapeutic potential.
Source: Asian Journal of Chemistry
Institution: Andhra University
Language: English
Subfield: Organic Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: diamond
OpenAlex ID: W4410788900