Year: 2025
Type: article
Abstract: Sister-chromatid cohesion mediated by the cohesin complex is critical for accurate chromosome segregation during mitosis. A key aspect of this process is the protection of cohesin at mitotic centromeres to resist spindle pulling-forces until anaphase onset. However, the mechanisms that prevent cohesin removal by its release-factor Wapl at centromeres remain incompletely understood. In this study, we identify ATRX, a chromatin remodeler of the SWI/SNF family, as a new binding protein of the cohesin complex. ATRX directly interacts with the cohesin accessory subunit Pds5B, antagonizing Wapl binding and thereby preventing premature release of centromeric cohesin. A mutation in ATRX that disrupts its interaction with Pds5B weakens centromeric cohesion and increases chromosome missegregation. Notably, centromere tethering of a Pds5B-binding fragment of ATRX, which lacks the ATPase domain, rescues cohesion defects in ATRX-depleted cells. Furthermore, Wapl depletion bypasses the requirement for ATRX, underscoring their antagonistic relationship. Together, these findings reveal a chromatin-remodeling-independent role for ATRX in maintaining centromeric cohesion by competitively inhibiting Wapl, providing new insights into the mechanisms that safeguard genomic stability.
Source: The EMBO Journal
Authors: Lei Zhao, Xueying Yuan, Qinfu Chen, Haiyan Yan, Fangwei Wang
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $7,990
Funders: National Natural Science Foundation of China, National Outstanding Youth Science Fund Project of National Natural Science Foundation of China, National Natural Science Foundation of China-Zhejiang Joint Fund for the Integration of Industrialization and Informatization, National Key Research and Development Program of China, Natural Science Foundation of Zhejiang Province
OpenAlex ID: W4410812333