Optimizing peptide detection using FET-based sensors: Integrating non-linearities of surface functionalization
Year: 2025
Type: article
Abstract: This study presents a method for enhancing peptide interaction with ATPES/linkers, utilizing tert -Butyloxycarbonyl to protect the N-terminal and prevent undesirable reactions. Addressing noise interference in peptide recognition, we devised a noise-filtering technique that enables accurate peptide signature retrieval even at low signal-to-noise ratio (SNR) by leveraging the full pH titration spectrum. Additionally, our research explores the impact of BOC protection levels on the functionalization of amine surfaces with APTES, highlighting how silanol sites influence the isoelectric point, surface potential, and capacitance. These findings underscore the necessity of considering chemical protection and surface chemistry in peptide synthesis and sequencing, offering valuable insights for bioanalytical applications.
Source: Solid-State Electronics
Authors: Naveen Kumar, Ankit Dixit, Prateek Kumar, Md. Hasan Raza Ansari, Navjeet Bagga, Navneet Gandhi, P.N. Kondekar, César Pascual García, Vihar Georgiev
Institutions: Dr. B. R. Ambedkar National Institute of Technology Jalandhar, University of Glasgow, King Abdullah University of Science and Technology, Indian Institute of Technology Bhubaneswar, Indian Institute of Information Technology Design and Manufacturing Jabalpur, Luxembourg Institute of Science and Technology
Language: English
Subfield: Bioengineering
Field: Chemical Engineering
Domain: Physical Sciences
Open Access status: hybrid
Estimated APC paid: $2,410
OpenAlex ID: W4410814457