Work

Germination fitness differs between penoxsulam resistant and susceptible Echinochloa phyllopogon biotypes

Year: 2025

Type: article

Abstract: : Echinochloa phyllopogon , a malignant weed that mostly reproduces through self-pollination in the paddy fields of Northeast China, currently presents critical resistance concerns. Multiple herbicide-resistant individuals were identified within the same population, and seed from each biotype were propagated for three generations. Three biotypes resistant to penoxsulam showed varying degrees of cross-resistance to other acetolactate synthase ( ALS ) inhibitors. The sequencing results indicated that the F4 generation seeds were homozygous with stably inherited mutations. A derived cleaved amplified polymorphic sequences (dCAPS) method is consistent with the sequencing results and can be used to quickly and accurately detect specific ALS mutations in E. phyllopogon . Sseed germination experiments revealed that R NTSR (non-target-site resistance) and R 197 biotypes exhibited significant germination fitness costs under certain extreme environmental conditions. At 200 mM NaCl concentration, the t E 50 values of R NTSR and R 197 were 11.37 and >14 days, respectively, with the mean germination times of 10.32 and 8.66 days, both longer than those of the sensitive (S) biotype. Under osmotic potential and soil burial conditions, the R NTSR biotype showed a reduced germination and emergence than the S biotype. Overall, the R NTSR and R 197 biotypes exhibit significant germination fitness costs under certain extreme environmental conditions, including temperature, salt concentrations, osmotic potential, and burial depths, compared with sensitive biotypes. This study elucidates the relationship between specific herbicide target enzyme mutations and weed germination fitness, and provides theoretical guidance for the control of resistant E. phyllopogon in the field.

Source: Agriculture Communications

DOI: 10.1016/j.agrcom.2025.100088

Authors: Haitao Gao, Jie Li, Jiaxing Yu, Yucheng Gu, Hongchun Wang, Liyao Dong

Institutions: Nanjing Agricultural University, Syngenta (United Kingdom), Jiangsu Academy of Agricultural Sciences

Language: English

Topic: Weed Control and Herbicide Applications

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDGs: Zero hunger, Life on land

Open Access status: gold

Estimated APC paid: $1,600