Work

Co-immobilization of levansucrase and endo-levanase on Immobead 150P support for fructooligosaccharide production

Year: 2025

Type: article

Abstract: Fructooligosaccharides (FOS) are commonly used as alternative sweeteners in healthy food and beverages due to their zero-calorie content and biologically beneficial activity.In this study, levan-type FOS were synthesized using co-immobilized levansucrase and endo-levanase.The enzymes were co-immobilized on Immobead 150P, which was activated with ethylenediamine and glutaraldehyde.The effects of immobilization time and enzyme concentration on immobilization efficiency and enzyme activity were investigated.Biochemical characterization revealed that the optimal pH and temperature for catalysis by both enzymes remained unchanged after immobilization.Moreover, the thermostability of both enzymes was significantly improved.FOS synthesis by the immobilized enzymes was examined using sucrose syrup as a substrate.The results showed that the immobilized enzymes could synthesize FOS with a yield of ∼45% across a broad range of pH (pH 3-7) and temperature (30-40 °C), indicating their potential for industrial applications.

Source: ScienceAsia

DOI: 10.2306/scienceasia1513-1874.2025.043

Authors: Thanapon Charoenwongpaiboon, Karan Wangpaiboon, Pawinee Panpetch, Rath Pichyangkura

Institutions: Silpakorn University, Chulalongkorn University

Language: English

Topic: Enzyme Catalysis and Immobilization

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Zero hunger

Open Access status: diamond

Funders: Silpakorn University, Thailand Science Research and Innovation, Faculty of Science, Silpakorn University