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Monitoring the Molecular Conformation of Individual Amphotericin B Molecules in an Aggregated State by Raman Optical Activity

Year: 2025
Type: article
Abstract: The polyene antifungal amphotericin B (AmB) can form giant helical aggregates. We show that microscopic and mesoscopic structural features of its aggregates can be revealed by Raman optical activity (ROA) and electronic circular dichroism (ECD), respectively. The ROA method, which senses molecular structure more locally, elucidates the conformation of the polyene chain of individual AmB molecules in an aggregated state. In turn, the ECD signal related to exciton coupling effects provides details about the arrangement of AmB molecules in the supramolecular structure. Thus, the use of both complementary methods will be crucial in future structural studies of chiral supramolecular systems.
DOI: 10.1021/acs.analchem.5c01198
Authors: Katarzyna Pajor, Grzegorz Zając, Marco Fusè, Marzena Mach-Liszka, Marta Arczewska, Mariusz Gagoś, Yoshimitsu Onaka, Tomotsumi Fujisawa, Masashi Unno, Malgorzata Baranska, Ewa Machalska
Institutions: Jagiellonian University, University of Brescia, University of Life Sciences in Lublin, Maria Curie-Skłodowska University, Saga University, Instytut Chemii i Techniki Jądrowej
Language: English
Subfield: Spectroscopy
Field: Chemistry
Open Access status: hybrid
Estimated APC paid: $4,500
Awards: G1421084007, Probing conformational changes in protein reaction sites via Raman optical activity, G3790789852, Investigations of the color tuning and photoreaction mechanisms for photoreceptor proteins by Raman optical activity, G6932654399, G7363488602, G7805551885
OpenAlex ID: W4410820479
FWCI:
Cites: 47
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Related to: 10