Work

Modeling Human Airway Epithelial Barrier Penetration Using Birch Bet v 1 and Alder Aln g 1 Pollen Allergens During Sensitization Process

Year: 2025

Type: article

Abstract: Pollen allergy is rated as a major public health problem, causing significant morbidity and adversely affecting the quality of people's lives. The airway epithelium serves as the first line of defense in the respiratory system, playing a crucial role in orchestrating immune responses to allergens. In this work, we studied the important transport steps in the major alder pollen allergen Aln g 1 through the human airway epithelium in comparison with those of the birch pollen allergen Bet v 1. Using fluorescence spectroscopy, we showed that both allergens can destroy liposomes with a composition modeling the adult human pulmonary surfactant. Using a polarized Calu-3 monolayer, we showed similar efficiencies of Aln g 1 and Bet v 1 transport through the artificial epithelial barrier. Using qPCR, we showed that Aln g 1 upregulates the expression of IL-33, TSLP, IL-1β, CXCL8 in epithelial cells, playing an important role in the sensitization process. The obtained results may improve our understanding of the primary sensitization mechanisms with the involvement of the PR-10 family of lipid-binding allergens.

Source: International Journal of Molecular Sciences

DOI: 10.3390/ijms26115169

Authors: Daria N. Melnikova, Andrey E. Potapov, Tatiana V. Ovchinnikova, Ivan V. Bogdanov

Institutions: Russian Academy of Sciences, Institute of Bioorganic Chemistry, Centre of Advanced Studies

Language: English

Topic: Allergic Rhinitis and Sensitization

Subfield: Immunology and Allergy

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,499