Sustained Oropouche virus transmission in Rio de Janeiro’s Atlantic Forest: genomic evidence over a two-year period
Year: 2026
Type: article
Abstract: BACKGROUND: Oropouche virus (OROV), an arbovirus endemic to the Amazon region, has recently expanded into non-endemic areas including Rio de Janeiro State, Brazil. OBJECTIVE: To characterise the spatio-temporal dynamics and ecological factors associated with OROV transmission in Rio de Janeiro during 2024-2025. METHODS: We analysed OROV case-associated ecological factors and performed a phylodynamic analysis on 40 viral genomes, comprising 35 new and five published sequences, sampled from 15 municipalities across the state during 2024-2025. FINDINGS: OROV cases showed significant positive correlations with forest area (r = 0.50, p < 0.0001), banana harvest area (r = 0.39, p < 0.01), and cassava harvest area (r = 0.29, p < 0.05); but these factors were autocorrelated, suggesting a confounded relationship. We identified two OROV sub-clades circulating in the Rio de Janeiro State. The OROVRJ/ES sub-clade was likely introduced into the Southern Fluminense region around January 2024, spread primarily by short (> 2 km, 50% of events) and mid-distance movements (2-9 km; 30%) with a mean dispersal rate of 0.3 km/day, and seed outbreaks in Metropolitan and Northwest Fluminense regions in 2025. The OROVES-I clade was likely introduced into Central Fluminense and Coastal Lowlands regions, later spreading to the Northern Fluminense region. MAIN CONCLUSIONS: Following its introduction in early 2024, OROV persisted in Rio de Janeiro State by spreading through short-distance movements among municipalities with high forest cover and agricultural areas. The sustained multi-year OROV transmission in the Atlantic Forest biome highlights the potential for establishment of endemic cycles beyond the Amazon region and the need for enhanced surveillance in extra-Amazonian areas, where OROV will evolve in a different ecosystem.
Authors: Ighor Arantes, Fernanda de Bruycker-Nogueira, Carla de Oliveira, Patrícia Carvalho Sequeira, Otília Lupi, Michele Borges, Carolina Lopes Melo, Michelle Brendolin, Antonio Braga, Luciane de Souza Velasque, Andréa Cony Cavalcanti, Adriana Cardoso Camargo, Fábio Burack da Costa, Cristiane Gomes de Castro Moreira, Vanessa Zaquieu Dias, Thiago de Jesus Sousa, Felipe Donateli Gatti, Gabriela Colombo de Mendonça, Joana Zorzal Nodari, Rodrigo Ribeiro-Rodrigues, Edson Delatorre, Guilherme Calvet, Felipe Gomes Naveca, Patricia Brasil, Ana Maria Bispo de Filippis, Gonzalo Bello
Language: English
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
Awards: G1118538860, G1153191922, G1307947927, G1675552654, G1840770272, G2171247466, G2584274330, G292321625, G3011539885, G3065519713, G3225036772, G3511672585, G3777054292, G4093845212, G4100756641, G4390702313, G4821847768, G4902303082, G5029353989, G5044813698, G5157461147, G5232883089, G5234577885, G5412096571, G5440400194, G5737600136, G6161906655, G6172025868, G6509747343, G6922052550, G7054501586, G7735966475, G8458562911, G8480654842, G8846401025, G943293303
OpenAlex ID: W7143528897