Year: 2025
Type: article
Abstract: Transcription factor forkhead box P1 (FOXP1) is a key regulator of immune cell functions. We have shown that FOXP1 contributes to the expansion of human hematopoietic stem/progenitor cell (HSPC) and acute myeloid leukemia cells. Here, we investigated the role of FOXP1 in early adult mouse hematopoiesis in vivo. We showed that loss of hematopoietic-specific FOXP1 expression leads to attrition of the hematopoietic stem cell (HSC) and multipotent progenitor (MPP)-1 compartment in parallel with enhancement of myeloid-biased MPP3 in adult bone marrow and fetal liver. Transplantation experiments confirmed that FOXP1-deficient bone marrow had an intrinsic reduced HSC compartment. FOXP1-deficient MPP compartments also showed enhanced proliferation with G0 phase reduction. Transcriptome analyses revealed that FOXP1-deficient HSC exhibited reduced stemness and enhanced expression of cell proliferation pathways. Thus, our current results revealed that FOXP1 plays a critical role in early murine hematopoiesis by maintaining HSCs, limiting the expansion of all MPP compartments, and restricting early myeloid commitment in vivo.
Source: Experimental Hematology
Authors: Françoise Levavasseur, Samia Oussous, Alessandro Framarini, Ismael Boussaid, Panhong Gou, Zubaidan Tuerdi, Iman Litchy Boueya, Helyette Hoffner, Marta De Almeida, Morgane Le Gall, Haley Tucker, Stéphane Giraudier, Didier Bouscary, Michaela Fontenay, Diana Passaro, Isabelle Dusanter-Fourt, Evelyne Lauret
Institutions: Centre National de la Recherche Scientifique, Inserm, Université Paris Cité, Institut Cochin, Hôpital Cochin, Assistance Publique – Hôpitaux de Paris, Hôpital Saint-Louis, Hématopoïèse normale et pathologique : Emergence, environnement et recherche translationnelle, The University of Texas at Austin
Language: English
Subfield: Hematology
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $3,250
Awards: G4553875974, G4733349049
OpenAlex ID: W4410858406