Development of a method for quantitative determination of total flavonoids in Astragalus Glycyphyllus L. herb
Year: 2025
Type: article
Abstract: Aim – to develop a method for the quantitative determination of the amount of flavonoids in the herb of Astragalus glycyphyllus. Material and methods. The object of the study was the herb of a perennial wild herbaceous plant, Licorice-leaved Astralagus herb, Astragalus glycyphyllus L., of the legume family Fabaceae, harvested in the Kursk region during 2023-2024. The raw materials were harvested independently during the mass flowering phase of the plant. With all the variety of methods for quantitative determination of the flavonoid content in plant raw materials, differential spectrophotometry was chosen as the most convenient and reproducible method. At the initial stage of the methodology development, the conditions for raw material extraction were determined, allowing for maximum extraction of flavonoids. Then, the most suitable conditions for complexation and spectrophotometry were selected empirically. Spectrophotometry itself was carried out on an SF-2000 spectrophotometer in the wavelength range from 350 to 450 nm. Results. The highest yield of flavonoids from the herb of Astragalus glycyphyllus can be achieved in the following conditions: the raw material is ground to 1 mm, the extraction agent is 70% ethyl alcohol, extraction is performed with heating in a boiling water bath for 45 minutes. The optimal conditions for the reaction of complexation with a chromogenic reagent are the use of 2 ml of a 5% solution of aluminum chloride. Stable coloration of extracts from raw materials with aluminum chloride occurs after 15 minutes and lasts for 90 minutes. Measurement of optical density were carried out at a wavelength of 405 ± 5 nm, which is the absorption maximum for this type of raw material. Since this value coincides with the absorption maximum of hyperoside with aluminum chloride, the hyperoside of Fitopanatseya LLC was used as a standard sample. As the results showed, the content of the sum of flavonoids in the herb of Astragalus glycyphyllus in terms of hyperoside averages 1.03 ± 0.041%, the error of a single determination with a probability of 95% does not exceed 4.51%. Based on these values, it is recommended to establish the standard of flavonoid content in the herb of Astragalus glycyphyllus of at least 1.0%. Conclusion. The developed method can be used to standardize the raw material of Astragalus glycyphyllus herb by the content of flavonoids, as the main group of active substances, when developing regulatory documentation and determining the commodity characteristics of the analyzed raw materials.
Source: Aspirantskiy Vestnik Povolzhiya
DOI: 10.35693/avp677190
Author: Tatyana A. Pozdnуakova
Institution: Orel State University named after I.S. Turgenev
Language: English
Subfield: Complementary and alternative medicine
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W4410860274