Work

Efficient Oxidative Polymerization of Hydroxytyrosol Using Horseradish Peroxidase Immobilized on a Magnetic Silica‐Coated Nanocatalyst

Year: 2025

Type: article

Abstract: Enzyme‐based catalysis has gained considerable attention in recent years due to its eco‐friendly and selective nature. However, the inability to recover enzymes after the reaction significantly increases operational costs. In this study, a reusable nanocatalyst Fe3O4@SiO2@APTES@GA@HRP is synthesized and applied in the oxidative polymerization of hydroxytyrosol. Fe3O4 nanoparticles are prepared using the solvothermal method, followed by silica coating via the Stöber process. Amino‐functionalization is achieved with 3‐aminopropyltriethoxysilane (APTES), and horseradish peroxidase (HRP) is immobilized through glutaraldehyde (GA)‐mediated azomethine bonding. The synthesized catalyst is characterized using SEM, EDS, FTIR, Q‐TOF, ¹H‐NMR, and Zetasizer analyses, all confirming successful immobilization. Polymerization reactions are conducted in acetate buffer (pH 5, 25 °C), resulting in a 73% yield. The catalyst is reusable for up to ten cycles, and the molecular weight of the produced poly(hydroxytyrosol) is approximately 30,000 g mol−1. These findings demonstrate the promising application of recyclable enzyme nanocatalysts in green polymer chemistry.

Source: Global Challenges

DOI: 10.1002/gch2.202500064

Authors: Fatih Sevim, Gülşah Aktaş, Hakan Kiziltaş, Fatih Demir, Taner Tekin

Institution: Atatürk University

Language: English

Topic: Enzyme Catalysis and Immobilization

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Responsible consumption and production

Open Access status: gold

Estimated APC paid: $3,810