Protocol for RNA interference in gene expression in adult insects of Triatoma infestans (Hemiptera: Reduviidae)
Year: 2025
Type: article
Abstract: RNA interference (RNAi) is a technique that enables post-transcriptional gene silencing in organisms. Here, we present a protocol for silencing the clock gene period ( per ) in adult insects of Triatoma infestans using RNAi. We describe steps for insect rearing, synthesis of double-stranded RNA (dsRNA) of target genes, and abdominal microinjection with dsRNA. We then detail procedures for collecting and extracting RNA from heads at different time points and evaluating silencing efficiency using quantitative reverse-transcription PCR (RT-qPCR). For complete details on the use and execution of this protocol, please refer to Córdoba et al. 1
Source: STAR Protocols
Language: English
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $1,550
Funders: Universidad Nacional de Córdoba, Consejo Nacional de Investigaciones Científicas y Técnicas, Secretaria de Ciencia y Tecnología - Universidad Nacional de Córdoba, Agencia Nacional de Promoción Científica y Tecnológica, Secretaría de Ciencia y Técnica, Universidad Nacional de Río Cuarto, Secretaría de Ciencia y Técnica, Universidad de Buenos Aires, Fondo para la Investigación Científica y Tecnológica
OpenAlex ID: W4410878624