Work

Protocol for analysis of miRNAs in human melanoma cells

Year: 2025

Type: article

Abstract: MicroRNAs (miRNAs) significantly contribute to melanoma plasticity, a key factor in metastasis and therapy resistance. Here, we provide a protocol for analysis of miRNAs in human melanoma cells. We describe two approaches for plating cells, either using cell chambers or attaching cells to microscopy slides by means of centrifugation. We first focus on cell pretreatment and staining procedures using the RNAscope Plus small RNA-RNA high-definition (RNAscope Plus smRNA-RNA HD) assay. We then detail the quantitative analysis of miRNA expression and co-localization.

Source: STAR Protocols

DOI: 10.1016/j.xpro.2025.103861

Authors: Evelyn Lattmann, Tessa Guggiari, Patrick Turko, Mitchell P. Levesque

Institutions: University of Zurich, University Hospital Zurich

Language: English

Topic: MicroRNA in disease regulation

Subfield: Cancer Research

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,550