Work

Marine microbial mock communities for validating rRNA gene amplicon sequencing

Year: 2025

Type: article

Abstract: Mock communities are defined mixtures of cells or DNA for methods development, testing, or validation. Here, we present mock communities based on previously used 16S and 18S rRNA gene sequences from diverse marine microorganisms. Their use as controls for amplicon sequencing enables the detection of biased or aberrant runs.

Source: Microbiology Resource Announcements

DOI: 10.1128/mra.00298-25

Authors: Robert H. Lampe, Ariel J. Rabines, Bryce A. Ellman, Hong Zheng, Andrew E. Allen

Institutions: J. Craig Venter Institute, Scripps Institution of Oceanography, University of California San Diego

Language: English

Topic: Microbial Community Ecology and Physiology

Subfield: Ecology

Field: Environmental Science

Domain: Physical Sciences

Open Access status: diamond

OpenAlex ID: W4410895179

FWCI: 0.532

Cites: 18

Cited by: 1

Related to: 10