Figure 4 from P4HA1 Mediates Hypoxia-Induced Invasion in Human Pancreatic Cancer Organoids
Year: 2025
Type: other
Abstract: Knockdown of P4HA1 decreases invasion in PDAC PDOs in hypoxia. A, Expression level of P4HA1 measured by qRT-PCR. Fold changes, expressed relative to the modified control samples, represent a mean of three technical replicates with SDs (PDO4 P = 0.0547, t test; PDO6 P = 0.0816, t test). B, Western blot analysis to confirm effectiveness of P4HA1 knockdown in PDO4 and PDO11 compared with the empty vector. C, Representative images of invasive shControl and shP4HA1_2-modified organoids in hypoxia. Scale bars, 100 μm. D, Effect of P4HA1 knockdown on invasion in modified PDO4 and PDO11 organoids. Percent invasion represents a mean of three biological replicates with SDs. Each biological replicate n > 30 organoids. (P = 0.0005 and P < 0.0001, t test). E, Inverse circularity scores of empty vector–modified and P4HA1 knockdown–modified PDO4 (top; n = 41 and n = 27, respectively; P = 0.0079, t test) and PDO11 (down; both n = 40; P < 0.0001, t test) organoids in hypoxia.
Authors: Bernat Navarro-Serer, Maria F. Wissler, Brandi K. Glover, Michael G. Lerner, Harsh H. Oza, Vania Wang, Hidur Knutsdottir, Fatemeh Shojaeian, Kathleen Noller, Saravana Gowtham Baskaran, Sarah Hughes, Alana M. Weaver, Daniel Wilentz, Oluwatobiloba Olayemi, Joel S. Bader, Elana J. Fertig, Daniele M. Gilkes, Laura D. Wood
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4410901028